Effects of Storage Temperature on Blood Components Using δ13C and δ15N SIA
Faculty Mentor
Karin Melkonian
Area of Research
Blood Storage SIA
Major
Biology
Description
Stable Isotopes are non-radioactive variants that facilitate the analysis of δ13C and δ15N across various elements. Through the application of stable isotope analysis in blood storage, this study aims to evaluate the preservation and stability of cellular components over a seven-month period in blood samples obtained from two spayed female dogs: a Doberman and a Rottweiler. We recorded empty and wet weight of the blood samples, which were stored at four different temperatures: -80°C, room temperature, refrigerator, and freezer. For δ13C and δ15N, storage in -80°C and room temperature seem to track each other in plasma and RBCs, which was predicted to be the farthest apart. The results indicate that there is not a significant difference in isotope ratios of the samples during the observed period. We anticipate that our findings will provide valuable insights into the effects of various storage methods and their impact on the integrity of blood samples, thereby informing future studies on blood sample preservation.
Effects of Storage Temperature on Blood Components Using δ13C and δ15N SIA
Stable Isotopes are non-radioactive variants that facilitate the analysis of δ13C and δ15N across various elements. Through the application of stable isotope analysis in blood storage, this study aims to evaluate the preservation and stability of cellular components over a seven-month period in blood samples obtained from two spayed female dogs: a Doberman and a Rottweiler. We recorded empty and wet weight of the blood samples, which were stored at four different temperatures: -80°C, room temperature, refrigerator, and freezer. For δ13C and δ15N, storage in -80°C and room temperature seem to track each other in plasma and RBCs, which was predicted to be the farthest apart. The results indicate that there is not a significant difference in isotope ratios of the samples during the observed period. We anticipate that our findings will provide valuable insights into the effects of various storage methods and their impact on the integrity of blood samples, thereby informing future studies on blood sample preservation.