Date of Award

2024

Document Type

Thesis

Degree Name

Master of Science in Biology

Department

Biology

Committee Chair and Members

Joseph Morin, Chair

Cecilia Kovac

Anthony Paratore

Keywords

Biology, Covid-19, Molecular biology, NSP3, Protein, Y2HGold

Abstract

The NSP3 protein of SARS-CoV-2 plays a crucial role in the virus-host interaction during infection. This thesis explores the protein-protein interactions between NSP3 and host factors, shedding light on their significance in viral replication and pathogenesis. NSP3 is known to interact with various host proteins involved in key cellular processes, including innate immune response modulation, RNA processing and protein degradation pathways. Understanding these interactions is essential for developing targeted therapeutic strategies against COVID-19. Application of The Matchmaker GAL4Two-Hybrid Systems, to isolate the NSP3 protein and study the interactions, elucidating the NSP3 host protein interactions and attempting to identify potential key proteins that may aid to future drug targets to disrupt viral replication and inhibit the progression of infection. Furthermore, blocking these interactions may offer a promising approach to attenuate viral pathogenicity and reduce the severity of COVID-19 symptoms. Moreover, the development of robust protocols to block NSP3-mediated viral replication holds significant potential for combating future SARS-CoV-2 outbreaks and other emerging coronaviruses. These protocols may involve the design of specific inhibitors targeting NSP3 enzymatic activities or critical interaction interfaces. Additionally, combination therapies targeting multiple viral proteins or host factors may enhance efficacy and reduce the likelihood of drug resistance development. Unraveling the NSP3 protein's interactions using the Yeast two hybrid system screening, and studying the revealed interactions will provide valuable insights into SARS-CoV-2 pathogenesis and offers promising avenues for the development of novel antiviral therapies. By targeting NSP3-mediated processes, researchers can advance the development of effective drugs and protocols to block viral replication and mitigate the impact of COVID-19 on global health.

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