Antibiotic Disk Synergy on M. Luteus
Faculty Mentor
Tara Leath
Area of Research
Microbiology
Description
INTRODUCTION: The rise of bacterial resistance has become a growing global concern, particularly within the medical field, as increasing resistance makes it more challenging to combat bacterial infections and develop effective antibiotic treatments.
METHOD: However, antibiotic synergy offers a promising route to improve inhibiting bacterial growth and mitigate resistance. By combining antibiotics with varying ratios, synergistic effects may be achieved to enhance overall antibiotic treatment efficacy. This study investigates how different ratios of chloramphenicol and tetracycline influence the growth of Micrococcus luteus through the use of double disk synergy. The effectiveness of the antibiotics chloramphenicol and tetracycline against Micrococcus luteus can be determined by comparing their individual effects to their combined impact through analysis of the zone of inhibition. Tetracycline works by stopping bacterial protein production when it binds to the 30S subunit, blocking transfer RNA from attaching properly. As a result, the bacteria become unable to produce essential proteins and therefore unable to grow and multiply. Chloramphenicol is similar, in that it also inhibits bacterial protein synthesis, except it binds to the 50S subunit, preventing peptide bond formation and stopping bacterial growth.
RESULTS: We believe that double disk synergy of tetracycline and chloramphenicol would result in larger inhibition zones, as opposed to the use of these antibiotics individually.
DISCUSSION/CONCLUSION: Each antibiotic targets a different subunit of the M. luteus ribosome, obstructing protein synthesis and bond formation, therefore resulting in greater inhibition when combined.
Antibiotic Disk Synergy on M. Luteus
INTRODUCTION: The rise of bacterial resistance has become a growing global concern, particularly within the medical field, as increasing resistance makes it more challenging to combat bacterial infections and develop effective antibiotic treatments.
METHOD: However, antibiotic synergy offers a promising route to improve inhibiting bacterial growth and mitigate resistance. By combining antibiotics with varying ratios, synergistic effects may be achieved to enhance overall antibiotic treatment efficacy. This study investigates how different ratios of chloramphenicol and tetracycline influence the growth of Micrococcus luteus through the use of double disk synergy. The effectiveness of the antibiotics chloramphenicol and tetracycline against Micrococcus luteus can be determined by comparing their individual effects to their combined impact through analysis of the zone of inhibition. Tetracycline works by stopping bacterial protein production when it binds to the 30S subunit, blocking transfer RNA from attaching properly. As a result, the bacteria become unable to produce essential proteins and therefore unable to grow and multiply. Chloramphenicol is similar, in that it also inhibits bacterial protein synthesis, except it binds to the 50S subunit, preventing peptide bond formation and stopping bacterial growth.
RESULTS: We believe that double disk synergy of tetracycline and chloramphenicol would result in larger inhibition zones, as opposed to the use of these antibiotics individually.
DISCUSSION/CONCLUSION: Each antibiotic targets a different subunit of the M. luteus ribosome, obstructing protein synthesis and bond formation, therefore resulting in greater inhibition when combined.